Fig. 3: VHHs A31 and Z70mut1 block the cellular uptake of recombinant tau microtubule binding domain (tauMTBD).
From: Inhibition of tau neuronal internalization using anti-tau single domain antibodies
Cellular tauMTBD uptake assay performed using 0.5 µM of recombinant monomeric tauMTBD labeled with the Atto488 fluorophore. a Percentage of cellular tauMTBD uptake based on Atto488 fluorescence signal quantification in the conditions resulting from tauMTBD co-incubation with each of the VHHs anti-GFP, A31, Z70mut1, E2-2, and H3-2 at a concentration of 1 µM. n = 3 independent experiments per condition. Data were normalized to the percentage of uptake relative to the condition of tauMTBD pre-incubated with PBS, defined as 100% of tauMTBD uptake. Box plots show the median (center line), 25th, 75th percentiles (box), and minimum and maximum values (whiskers), all data points are shown (single points). Data were analyzed using one-way nonparametric ANOVA (Kruskal-Wallis) with Dunn’s multiple comparison test (****p < 0.0001; **p < 0.01). VHHs A31 and Z70mut1 (p = 0.0061) significantly reduced cellular tau uptake. b Confocal microscopy analysis of cellular tauMTBD uptake assay under the conditions described in (a) (images are representative of a n = 2 independent experiments per condition). TauMTBD is visualized in green, and nuclei in light blue. The scale bar is 10 µm.
