Fig. 4: Cellular internalization of tau fibrils is inhibited by the anti-tau VHH H3-2.
From: Inhibition of tau neuronal internalization using anti-tau single domain antibodies
a Inhibition of cellular uptake of fibrillar tau1N4R by the anti-tau VHHs. Percentage of cellular tau fibril uptake based on Atto565 fluorescence signal coming from tau1N4R fibrils co-incubated with PBS (tau_F), RAP (400 nM), heparin (50 µg/mL), and the VHHs (400 nM). n = 3 independent experiments per condition. Data were normalized to the condition of tau1N4R fibrils pre-incubated with PBS as 100% of tau uptake. Box plots show median (center line), 25th, 75th percentiles (box,) and minimum and maximum values (whiskers), all data points are shown (single points). Data were analyzed using one-way nonparametric ANOVA (Kruskal-Wallis) with Dunn’s multiple comparison test (****p < 0.0001; **p < 0.01). Heparin and VHH H3-2 (p = 0.0096) significantly reduced cellular tau fibril uptake. b Confocal microscopy analysis of fibrillar tau cellular assays using recombinant tau1N4R fibrils labeled with Atto565 dye and in the presence of PBS (tau_M), RAP, heparin, and VHHs A31, Z70mut1, or H3-2 (images are representative of a n = 2 independent experiments per condition). Tau was visualized in red, and nuclei in light blue. The scale bar is 10 µm.
