Table 2 Table summarizing the affinity parameters of the interaction between the tau[369−381] C-terminal peptide and VHHs E2-2 or H3-2

From: Inhibition of tau neuronal internalization using anti-tau single domain antibodies

VHH

Kinetic analysis

Steady-state analysis

kon (M−1.s−1)*10+2

koff (s−1)*10-3

KD (nM)

KD (nM)

SR

E2-2

1540 ± 271

124 ± 13

820 ± 100

578 ± 213

0.45

H3-2

1918 ± 359

71 ± 2

280 ± 70

297 ± 84

0.41

  1. Kinetic kon and koff, and KD values are included in the table. For the steady-state analysis, KD were determined using the one-site specific binding model. The stoichiometric ratio (SR) was calculated from the ratio of the experimental Rmax to the theoretical Rmax.
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