Fig. 1: Comparative analysis of T. brucei proximal and distal-specific axoneme proteins in L. Mexicana.revealed multiple localisation groups.
From: Proteins with proximal-distal asymmetries in axoneme localisation control flagellum beat frequency
A–D Quantitative analysis of fluorescence signal distribution along the axoneme from proximal and distal-specific axoneme proteins endogenously tagged with mNG at the N and/or C terminus. Each row corresponds to a T. brucei protein and its L. mexicana ortholog. In the first (A, B) and third columns (C, D), are an example T. brucei and L. mexicana cell, respectively. Phase contrast (grey), DNA (Hoechst 33342, magenta) and mNG (green) overlay (left) and mNG fluorescence (right) are shown. In the second (A, B) and the fourth columns (C, D), graphs representing the mNG fluorescence signal intensity along the axoneme, from the base to tip. Proteins are grouped by proximal (A, C) and distal (B, D) localisations. Data points represent the mean of n = 15 axonemes in 1K1N cells, normalised by maximum signal intensity per cell. Source data are provided in the Source Data file.
