Fig. 4: Proximal and distal-specific localisations can be either DC asymmetry dependent or independent.
From: Proteins with proximal-distal asymmetries in axoneme localisation control flagellum beat frequency
Protein localisation changes on pDC1 or dDC2 deletion. In the first column micrographs of L. mexicana cell line expressing tagged proximal and distal proteins. Second column: after deletion of both alleles of pDC1. Third column: after deletion of dDC2. Phase contrast (grey), DNA (Hoeschst 33342, magenta) and mNG (green) overlay and mNG fluorescence are shown. Tagged proteins are grouped by being a DC1 or 2 ortholog and by proximal or distal localisation. A pDC1 and 2. B Other proximal localising proteins. C dDC1 and 2. D Other distal localising proteins. Cell lines where the protein fails to localise to the axoneme are outlined in orange, and cell lines where the localisation is axonemal but changed are outlined in blue. Increased length of proximal signal are highlighted with a circle.
