Fig. 2: PACT inhibits PKR phosphorylation during RNA viral infection. | Nature Communications

Fig. 2: PACT inhibits PKR phosphorylation during RNA viral infection.

From: Cooperative role of PACT and ADAR1 in preventing aberrant PKR activation by self-derived double-stranded RNA

Fig. 2

A, B U2OS (A) or A549 (B) WT and PACT KO cells were infected with SeV (MOI = 1, 24hpi). The levels of PKR phosphorylation (PKR-pT446) and other indicated proteins were monitored by western blot. C U2OS WT and PACT KO cells were transfected with 200 ng/mL of poly(I:C) for 6 h. The levels of PKR-pT446, PACT, and Vinculin were monitored by western blot. D, E U2OS WT and PACT KO cells were infected with PV (MOI = 1, 20hpi) (D) or EV-A71 (MOI = 5, 24hpi) (E). The levels of the indicated proteins were monitored by western blot. F The levels of PKR-pT446, eIF2α-pS51, SeV, PACT, and Vinculin were analyzed in the indicated U2OS cell lines by western blot after SeV infection (MOI = 1) at 24hpi. PACT-HA was expressed when indicated. Source data are provided as a Source Data file.

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