Fig. 5: Loss of PACT triggers synthetic lethality in ADAR1-deficient cells.
A Schematic representation of CRISPR-dropout screening approach. PD; population doubling. Created in BioRender. Buisson, R. (2025) https://BioRender.com/f77e526. B Dot plots graph representative of the genes depleted in PACT KO cells analyzed using the MaGeCK computational tool. Each dot represents a unique gene. ADAR1 gene was found to be significantly depleted in PACT KO cells and was highlighted in purple. C, D Crystal violet staining showing the viability of U2OS (C) or A549 (D) WT or PACT KO cells transfected with siRNA control (siCTL) or against ADAR1 (siADAR1). Cells were stained with Crystal violet 6 days following transfection with siRNA. E U2OS WT or PACT KO cells were knocked down with siCTL or siADAR1. Cell survival was quantified with Alamar blue cell viability assay 6 days following siRNA transfection. Mean values ± SD (Number of biological replicates, n = 3). P-values were calculated by one-way ANOVA. F Crystal violet staining showing viability of U2OS WT, PACT KO, PACT KO + PACTWT, or PACT KO + PACTEA cells transfected with siRNA control (siCTL) or against ADAR1 (siADAR1). Cells were stained with Crystal violet 6 days following transfection with siRNA. G Indicated cell lines, expressing when indicated PACTWT or PACTEA were knocked down with siCTL or siADAR1. Cell survival was quantified with Alamar blue cell viability assay 6 days following siRNA transfection. Mean values ± SD (Number of biological replicates, n = 3). P-values were calculated by two-way ANOVA. Source data are provided as a Source Data file.
