Fig. 6: Loss of PCLO significantly impaired the ccRCC progression. | Nature Communications

Fig. 6: Loss of PCLO significantly impaired the ccRCC progression.

From: Identifying potential risk genes for clear cell renal cell carcinoma with deep reinforcement learning

Fig. 6

A, B The protein and mRNA expression of PCLO after transient transfection of shPCLO lentivirus in 293T cells. n = 3 independent samples. C The protein expression of PCLO in cells of 786-O-shPCLO. D The cell viability of 786-O-shPCLO cells. n = 10 from three biological replicates. E–H The assays of apoptosis, colony formation, migration, and invasion in 786-OshPCLO cells. n = 3 independent experiments. I, J Average morphology of 786-O-shPCLO cells per well, including cell width, length, area, and perimeter, was evaluated at multiple time points using a high-content cell imaging analysis system. n = 10 independent samples. K, L Average movement speed and accumulated distance of 786-O-shPCLO cells per well. n = 10 independent samples. NC negative control, ns no significant. The data were shown as the mean  ± SEM. One-way ANOVA (B). Two-tailed unpaired Student’s t-test (D–L). Source data are provided as a Source Data file.

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