Fig. 3: Nap1l1 p.D349E exacerbated cardiac hypertrophy via cGas-Sting signaling in mouse with Ang II treatment.

A Schematic diagram depicting the experimental strategy used in C57BL6/N mice. Created in BioRender. Lv, C. (2025) https://BioRender.com/w55g327. B, C Representative image of heart and wheat germ agglutinin (WGA) staining and quantification of the WT, MT, WT with Ang II and MT with Ang II groups. N = 19, 20, 12, and 10 for WT, MT, WT with Ang II and MT with Ang II groups, and one-way ANOVA with Tukey tests were used for correction of multiple comparisons; D Heart weight-to-body weight (HW/BW) ratios of the WT, MT, WT with Ang II and MT with Ang II groups. N = 8 for each group, and one-way ANOVA with Tukey tests was used for correction of multiple comparisons; E Heart weight-to-tibia length (HW/BW) ratio in the WT, MT, WT with Ang II and MT with Ang II groups. N = 8 for each group, and one-way ANOVA with Tukey tests was used for correction of multiple comparisons; F Representative hematoxylin and eosin (H&E) staining showing preserved heart size in the WT, MT, WT with Ang II and MT with Ang II groups. G Representative images of interstitial fibrosis stained by Masson’s trichrome staining showing preserved heart size in the WT, MT, WT with Ang II, and MT with Ang II groups. H Representative M-mode echocardiograms of the left ventricle in the WT, MT, WT with Ang II, and MT with Ang II groups. I Quantification of left ventricular end-diastolic anterior wall thickness (LVAW; d), left ventricular end-diastolic posterior wall thickness (LVPW; d), ejection fraction (EF), fractional shortening (FS) or left ventricular (LV) mass; J qPCR analysis of Anp, Bnp, and β-Mhc in the WT, MT, WT with Ang II and MT with Ang II groups. N = 5 for each group, and one-way ANOVA with Tukey tests was used for correction of multiple comparisons; K Relative concentration of second messenger cGAMP. N = 5 for each group, and one-way ANOVA with Tukey tests was used for correction of multiple comparisons; L qPCR analysis of Ifna and Ifnb in the WT, MT, WT with Ang II, and MT with Ang II groups. N = 5 for each group, and one-way ANOVA with Tukey tests were used for correction of multiple comparisons; M, N Western blot images and quantification showing cGAS, Sting, Tbk1, Ifna, and Ifnb expression and phosphorylation of Sting, Tbk1, and Irf3 in the WT with Ang II and MT with Ang II groups. MT: mutation type, NAP1L1 p.D349E. N = 5 for each group and one-way ANOVA with Tukey tests was used for the correction of multiple comparisons. Data are presented as mean values +/− SEM.