Fig. 5: cGas-Sting-IFN blockade alleviated NAP1L1 p.D349E-induced cardiac hypertrophy.
From: Somatic NAP1L1 p.D349E promotes cardiac hypertrophy through cGAS-STING-IFN signaling
A Schematic diagram depicting the experimental strategy used in C57BL6/N mice with antagonists and antibodies. C-176, a selective STING antagonist. Created in BioRender. Lv, C. (2025) https://BioRender.com/u41l117. B Representative image of heart and wheat germ agglutinin (WGA) staining and quantification of WT with AngII, MT with AngII, MT with AngII and C-176, and MT with AngII and anti-IFNAR groups; N = 10, 8, 9, 12 for WT with AngII, MT with AngII, MT with AngII and C-176, and MT with AngII and anti-IFNAR and one-way ANOVA with Tukey tests was used for correction of multiple comparisons; C Representative hematoxylin and eosin (H&E) staining and Masson’s trichrome staining showing heart size, cardiomyocyte size, and fibrosis in WT with AngII, MT with AngII, MT with AngII and C-176, and MT with AngII and anti-IFNAR groups; D Heart weight to body weight (HW/BW) ratio in WT with AngII, MT with AngII, MT with AngII and C-176, and MT with AngII and anti-IFNAR groups. N = 8 for each group, and one-way ANOVA with Tukey tests were used for correction of multiple comparisons; E Representative M-mode echocardiograms of the left ventricle in WT with AngII, MT with AngII, MT with AngII and C-176, and MT with AngII and anti-IFNAR groups; F Quantification left ventricular end-diastolic anterior wall thickness (LVAW;d), left ventricular end-diastolic posterior wall thickness (LVPW;d), Ejection fraction (EF), Fractional shortening (FS) or left ventricular (LV) mass. N = 8 for each group, and one-way ANOVA with Tukey tests were used for correction of multiple comparisons; G Western blot analysis showing the cGAS, Sting, Tbk1, Ifna, and Ifnb, and phosphorylation of Sting, Tbk1, and Irf3 expression in WT with AngII, MT with AngII, MT with AngII and C-176 groups; H Representative Western blot images and quantification showing the cGAS, Sting, Tbk1, Ifna, and Ifnb, and phosphorylation of Sting, Tbk1 and Irf3 expression in WT with AngII, MT with AngII, MT with AngII and C-176 groups. MT: mutation type, NAP1L1 p.D349E. N = 5 for each group, and one-way ANOVA with Tukey tests was used for correction of multiple comparisons; I Central Illustration of this study. Created in BioRender. Lv, C. (2025) https://BioRender.com/g82u862. Data are presented as mean values +/− SEM.
