Fig. 3: Mutants lacking flp-13 and its receptor dmsr-1 display reversible quiescence upon PA14 infection.

A PA14-induced quiescence and death in frpr-4(ok2376) and dmsr-1(qn45) after 20 h of infection. B PA14-induced quiescence and death in indicated genotypes after 20 h of infection. C Time course of dmsr-1 behavior and viability, displayed as in (2 A). Error bars indicate standard deviation. For each genotype, n = 10 animals per replicate, 6 replicates. **p < 0.005, two-tailed Mann–Whitney test: Median quiescence observation was 20 hrs in dmsr-1 vs 60 hrs in WT animals (Fig. 1A). *p < 0.05, two-tailed Mann–Whitney test: Median time point at which 50% animals died was 48 hrs in dmsr-1 mutants vs 72 h in WT (Fig. 1A). D Representative images of expression of dmsr-1 transgene (syb6591) overlaid with NeuroPAL after exposure to OP50 or PA14 for 20 h. No differences were found. See Fig S3 for quantification. Quantification of fluorescent intensities of (E) hsp-6::GFP (F) hsp-4::GFP, or (G) irg-1::GFP, measured 6 h after being placed on indicated bacteria. n are individual animals and are indicated on the figure. *p < 0.05, ** p < 0.01, ***p < 0.001, n.s. indicates not significant (p > 0.05) with Wilcoxon rank sum test. Error bars indicate standard deviation. H Locomotion speed while blue light (0.5 mW) was applied to activate pdfr-1::BlaC. flp-13 animals lacking pdfr-1::BlaC provide a control for the generic effects of blue light on behavior. n = 27-63 tracked animals. Wilcoxon rank sum test (p < 0.0001) was run on n = 17 (flp-13;pdfr::BlaC), and n = 19 (flp-13) tracked animals in the 5 min period immediate after blue light onset. I Pumping rate of quiescent animals before and after blue light (0.5 mW) exposure for 10 s. n’s are individual animals and are indicated on the figure.***p < 0.001, Paired t test. J PA14-induced quiescence and death in indicated genotypes after 20 h of infection. For (A), (B), and (J) error bars represent the 95% confidence interval for the bootstrapped mean fraction values of animals. Shown data was collected across multiple days but statistical comparisons were computed between day-matched experimental groups. ***p < 0.001, **p < 0.01, fraction quiescence compared via Chi-square test with Bonferroni correction. Source data are provided as a source data file.