Fig. 3: Post-modification reactions for the biosynthesis of furochromones.

Functional characterization of SdCH (a), SdOMT (b), and SdUGT1/2 (c, d). Shown are HPLC/UV chromatograms of the enzyme catalysis reactions (λ = 280 nm), together with (+)-ESI-MS and MS/MS spectra of the products. e Kinetic parameters of SdUGT1 and SdUGT2. f Simulated binding modes of 8 in the crystal structure of SdUGT2 when catalyzing 11-O-glycosylation (left) and 3′-O-glycosylation (right). Hydrogen bonds and hydrophobic interactions were labeled with blue and orange dashes, respectively. STD, reference standard. Control, reaction mixtures incubated with boiled enzymes or microsomes.