Fig. 5: Immunization reduces expression of pro-inflammatory genes in mice post-challenge. | Nature Communications

Fig. 5: Immunization reduces expression of pro-inflammatory genes in mice post-challenge.

From: Intranasal influenza virus-vectored vaccine offers protection against clade 2.3.4.4b H5N1 infection in small animal models

Fig. 5

A Illustration of schedule of immunization, virus challenge and sacrifice for BALB/c mice. B Mice were intranasally vaccinated with DelNS1-cH5N1 (106 pfu), or PBS (n = 4 for each group), then challenged with cattle H5N1 virus (5000 pfu) 4 weeks after immunization. Pro-inflammatory genes were measured by RT-qPCR after isolation of RNA from lungs at 4 dpi. C Mice were intranasally vaccinated with DelNS1-mH5N1 (106 pfu), or PBS (n = 4 for each group), then challenged with mink H5N1 virus (104 pfu) 4 weeks after immunization. Pro-inflammatory genes were measured by RT-qPCR after isolation of RNA from lungs at 4 dpi. Data represent mean values ± SD of results. Statistical analysis was performed using Student’s t-test (two-tailed). The mouse cartoon was created in BioRender. Wang, P. (2025) https://BioRender.com/n83i107. Source data are provided as a Source Data file.

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