Fig. 5: Detection of post-translational modifications (PTMs) by single biological nanopore.
From: Nanopore sensing of protein and peptide conformation for point-of-care applications
a Left, sketch representing the most common PTMs. Right, the overall principle of PTM identification using nanopore technology. b1 Characterization and discrimination of Tau peptides according to different phosphorylation patterns in an engineered aerolysin nanopore (T232K/K238Q) in 1 M KCl, 10 mM Tris, 1 mM EDTA, pH8. Figure adapted from Small Methods, Li et al.27 © 2020 WILEY_VCH Verlag GmbH & Co. KGaA, Weinheim27 b2 Current traces showing the identification of different post-translational modifications of α-synuclein peptides with an aerolysin nanopore in 1 M KCl, 10 mM Tris, 1 mM EDTA, pH 7,4. Figure adapted from ACS nano, Cao et al.127. c Discrimination between unphosphorylated and phosphorylated biological peptides (FPA and FPA-P) with an Aerolysin nanopore. Different blockade levels are observed for each peptide, enabling their identification in the mixture. In addition, due to the high sensitivity of the aerolysin nanopore, two conformations were identified for the phosphorylated peptide. Figure adapted from ACS Central Science, Stierlen et al.25. Created in BioRender. Ratinho, L. (2025) https://BioRender.com/j25c673.
