Fig. 2: The TdLN supplies the tumor with PD-1+ TCF-1+ CD8+ T cells following RT + αPD-L1.

a Representative plots of PD-1+ GP33+ T cells and PD-1+ TCF-1+ cells vs. TIM-3+ in the tumor and its TdLN. b Quantitation of the PD-1+ TCF-1+ cells frequency in the tumor and its TdLN. Combined data from 2 experiments (n = 10 total). c Experimental schema with yellow bar representing FTY720 administration in drinking water. d Representative plots gated on CD8 showing PD-1+ GP33+ T cells in the tumor under different treatment conditions with or without FTY720. e Quantitation of the number of GP33+ T cells per gram tumor. Combined data from 2 experiments (n = 10 total). f Representative plots gated on antigen specific subsets under different treatment conditions with or without FTY720. g Quantitation of the number of antigen specific PD-1+ TCF-1+ T cells per gram tumor. Combined data from 2 separate experiments (n = 10 total per group). h Quantitation of the number of antigen specific PD-1+ TIM-3+ cells per gram tumor. Combined data from 2 separate experiments (n = 10 total per group). i Tumor kinetics under different treatment conditions with and without FTY720. Statistical significance calculated by two-tailed unpaired t tests. Combined data from 2 experiments (n = 15 total). All data are presented as mean values ± SEM. Statistical significance calculated by Kruskal-Wallis test, unless otherwise noted. Source data are provided as a Source Data file.