Fig. 3: Aged patients have a diminished pool of naïve T and B cells and higher NK cells pre- and post-treatment compared to young patients. | Nature Communications

Fig. 3: Aged patients have a diminished pool of naïve T and B cells and higher NK cells pre- and post-treatment compared to young patients.

From: Age-related divergence of circulating immune responses in patients with solid tumors treated with immune checkpoint inhibitors

Fig. 3

A Heatmap showing scaled expression of each cluster identified after CyTOF was performed on pre and post-treatment peripheral blood mononuclear cell (PBMC) samples with a 37-marker panel. A FlowSOM algorithm was used to generate metaclusters which were annotated into a final 15 clusters (immune groups). Scaled expression profile for each cluster is shown. B UMAP plots visualizing the annotated clusters (500 cells per sample). Proportion of the total cells selected major immune groups at (C) baseline and (D) on treatment (total n = 91, aged n = 49, young n = 42). In (C, D), the box and whisker plots show the median, interquartile range (IQR), minimum/maximum values, and additional marking of outliers. Statistical comparisons between quantitative measurements were performed using a two-sided Wilcoxon rank-sum test without adjustment for multiple comparisons. Source data are provided as a Source Data file.

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