Fig. 1: MetaFlowTrain system overview.

a MetaFlowTrain supports diverse inputs, including artificial media (e.g., TSB, ARE) and natural extracts (e.g., soil extracts, host exudates). b The system operates up to 24 trains per peristaltic pump with adjustable flow rates. c A modular setup allows for up to six microchambers per train. d Outputs include microbial and exometabolite collection for OD measurements, qPCR, amplicon sequencing, metabolomics, and plant growth assays. A movie describing the system is also available (Supplementary Movie 1).