Fig. 5: Effect of antibiotic-induced microbiota alterations on intestinal metabolome. | Nature Communications

Fig. 5: Effect of antibiotic-induced microbiota alterations on intestinal metabolome.

From: Maternal gut microbiota influences immune activation at the maternal-fetal interface affecting pregnancy outcome

Fig. 5

PCA of fecal metabolome in vancomycin-treated (green) and control (blue) dams at ed14.5 (n = 6, a). Box and Whisker plot representation of the metabolites showed a VIP-score >1.5 (n = 6 experiments, b). The plot shows all individual data points. Horizontal lines within the plot indicate the median values. Microbe-metabolite correlation heatmap suggesting associations between bacterial phyla and VIP-score selected metabolites at ed14.5. Spearman correlation coefficients were calculated for pairwise combinations of microbial abundances at the phyla level from 16S rRNA gene sequencing data and metabolite intensities (c). Pathway impact (d) and Metabolite Set Enrichment Overview (e) of vancomycin-treated dams normalized to control group at ed14.5. Global test algorithm was employed for both pathway impact and pathway enrichment analysis, Metaboanalyst3.0 (http://www.metaboanalyst.ca). Each measurement was taken from a distinct experiment. See also Supplementary Fig. 11. Source data are provided as a Source Data file.

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