Fig. 4: The mechanisms for ShosT-mediated toxicity and ShosA-mediated anti-toxicity.
From: A toxin-antitoxin system provides phage defense via DNA damage and repair
a Cell growth curves of E. coli BL21(DE3) strain expressing ShosT. Empty vector is used as the control. Results of three experiments are presented as the average of three replicates with shaded areas indicating SD. The time points of the drop of cell growth (blue) and cell collection (purple) are marked. Source data are provided as a Source Data file. b Diagram of the differential metabolite classification (KEGG). c Bubble map of KEGG pathway enrichment (up to top 5). d Fluorescence microscopy of E. coli BL21(DE3) cells expressing the whole system ShosTA, ShosT inactive mutant (D331A/D332A) or wild type ShosT. This experiment is repeated three times independently with similar results. Scale bars: 5 μm. e IP-MS/MS analysis reveals in vivo interactions between ShosA and some recombination-associated proteins. FLAG-tagged ShosA in the context of the full ShosTA system or FLAG-tag alone are used. Bar graphs represent the mean of three replicates with individual data points overlaid. f ShosA rescues E. coli BL21(DE3) cells from the toxicity of ccdB. Left, representative plating assay for indicated plasmids. Right, quantification of the left panel. Bar graphs represent the mean of four replicates with individual data points overlaid. Source data are provided as a Source Data file.
