Fig. 2: Mechanical overload- (MOV) induced muscle growth is not altered in HSA-MycKO mice. | Nature Communications

Fig. 2: Mechanical overload- (MOV) induced muscle growth is not altered in HSA-MycKO mice.

From: Muscle fiber Myc is dispensable for muscle growth and its forced expression severely perturbs homeostasis

Fig. 2

A Schematic of MOV surgery. B Plantaris (PLA) muscle mass normalized to body mass and the mean mass of the contralateral muscle after 3 (CON only; n = 3), 7, 14 and 28 days of MOV in CON (n = 4, 3, 7) and HSA-MycKO (n = 3, 3, 7) mice. C Digital PCR for transcript levels of Myc and Rpl3 normalized to a housekeeping gene (Hprt) in CON (HSAwt/wt; Mycfl/fl) and HSA-MycKO (HSACre/wt;Mycfl/fl) mice after 7 (CON, n = 4; KO, n = 3) and 14 (n = 3) days. D Representative images of RNA fluorescent in situ hybridization (RNA-FISH) with probes against Myc (magenta) and counterstaining with DAPI (blue) and laminin β1γ1 antibodies (white), for 3 (CON only), 7 and 14 days of MOV for CON and HSA-MycKO mice. E Quantification of Myc + (≥3 Myc puncta) nuclei outside laminin-stained muscle fibers (Non-muscle), Pax7+ muscle stem cell nuclei (MuSC) and peripherally (Myofiber) and centrally located (Central nuclei) nuclei within laminin stained muscle fibers, in contralateral and after 7 days of MOV (MOV-7d) in CON (n = 4) and HSA-MycKO (n = 3) mice. F Type-specific (IIA, IIX, IIB) fiber number in complete PLA cross-sections normalized to the contralateral PLA in CON (n = 6) and HSA-MycKO (n = 7) mice after 28 days of MOV and (G) representative images after 14 and 28 days of MOV. H Type-specific (IIA, IIX, IIB) fiber size distribution normalized to contralateral total fiber number (n = 7; or 6 for CON MOV-28d). I Quantification and (J) representative images of Pax7+ nuclei co-stained with <3 (low) 3–5 (Pos) or >5 (High) RNA puncta for Myc (upper) and MyoD (lower) using RNA-FISH in the contralateral muscle and after 7 days MOV (CON, n = 4; KO, n = 3). A Created in BioRender. Ruegg, M. (2025) https://BioRender.com/d74q573. Data are presented as mean ± SEM. Two-way repeated measure ANOVAs (A) or two-way ANOVAs with Sidak’s post hoc test were used to compare between data. *, **, and *** denote a significant difference between groups of P  <  0.05, P  <  0.01, and P  <  0.001, respectively.

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