Fig. 6: DOLs are found in other ALS mouse models. | Nature Communications

Fig. 6: DOLs are found in other ALS mouse models.

From: Correction of dysregulated lipid metabolism normalizes gene expression in oligodendrocytes and prolongs lifespan in female poly-GA C9orf72 mice

Fig. 6

a Heatmap of DOL marker genes and essential cholesterol pathway genes from bulk RNAseq across three mouse models of ALS confirms induction of DOLs and the disturbance of cholesterol pathway. Asterisks indicate significant changes (adjusted p < 0.05). (n as Fig. 1a for GA-Nes and rNLS8 mice. GA-Camk2a hippocampi from 5 control vs 6 transgenic mice. GA-Camk2a neocortex from 4 control vs 6 transgenic mice). b, c SerpinA3N staining in rNLS mice shows significant expression of Serpina3n mRNA in the transgenic condition. n: WT = 4, Tg = 5 from independent biological replicates. (Welch’s T-test) d, e SerpinA3N staining in GA-Camk2a mice shows significant expression of Serpina3n mRNA in the transgenic condition. n: WT = 4, Tg = 4 from independent biological replicates (Mann–Whitney U-Test). f PLIN4 (green) immunofluorescence co-stained with DAPI and oligodendrocyte marker CA2 (red) in Camk2a (control) and GA-Camk2a (transgenic) mice, respectively, confirms upregulation of PLIN4 in transgenic mice, both in oligodendrocytes as well as elsewhere. The shown images are adjusted for brightness and contrast in the same way for each channel in similar ways across both conditions. White arrow indicates an example of a CA2+ oligodendrocyte, yellow arrow shows an example of axon tracts, while magenta arrow highlights an example of PLIN4 in the neuropil. Images are representative of three animals. Scale bar = 50 µm.

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