Fig. 2: Observation of SCN neuronal light responses in awake mice. | Nature Communications

Fig. 2: Observation of SCN neuronal light responses in awake mice.

From: Discrete photoentrainment of mammalian central clock is regulated by bi-stable dynamic network in the suprachiasmatic nucleus

Fig. 2

a, b An illustration of recording setup and surgical procedure. c. Representative two-photon in vivo GCaMP images (averaged) of SCN taken at different ZT times through the GRIN endoscope. d Representative 3D-projection of SCN GCaMP signal using z-stack images through the GRIN endoscope. The XY projection shows the summation of horizontal planes, while the XZ projection shows the summation of sagittal planes of the SCN. The XZ virtual 45-μm-thick section demonstrates the elongated point spread function in the z-axis through GRIN endoscopes. e Schemes for time points experiments (upper panel) and calcium imaging recording (lower panel). f Representative images of GCaMP and tdTomato signals collected simultaneously with fast-scanning two-photon system. Arrows indicate the colocalization of both channels. g Averaged light-response of identified neurons at 3 ZT times. Red: ZT 22, green: ZT 8, blue: ZT 16. n = 3 animals. Scale bars are 100 μm, according to the imaging side of the GRIN endoscope. Outline of mouse brain in b was drawn according to Paxinos and Franklin’s the Mouse Brain79.

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