Fig. 5: Dynamic light responses from individual SCN neurons that form distinct temporal circuit.
a Percentage of inhibitory clusters (Cluster 7 and C6 - 7) is significantly decreased at ZT 8. Error bars indicate mean with SEM. b. Heat map of combined normalized traces from three-time points for 113 identified neurons ranked by mean Z-score at ZT16. c Representative heat maps of Pearson correlation coefficients comparing light responses between neurons. Left panel shows comparison between different ZTs, right panel shows comparison between different trials within the same ZT. Blue outlines indicate same neurons in different trials, and yellow outlines indicate different neurons in same trials. d Cross-time correlation analysis from each neuron between different trials (R-cross, blue outline in c and blue plots in d) is significantly lower than the correlation coefficients for different neurons at the same ZT time within a single trial (R-same, yellow outline in c and orange plots in d). e Representative diagram showing highly correlated neuron pairs (average r from 9 repeats > 0.5) from each time points. f. Scatter plots of distance between pairs of neurons and their Pearson correlation coefficient in respective time points. The r2 value of linear regression lines is 0.007, 0, and 0.004 for ZT 16, ZT 22, and ZT 8, respectively. For (a) and (d), numbers indicate p-value with one-way ANOVA, Tukey post hoc tests. Scale bars are 100 μm, according to the imaging side of the GRIN endoscope. n = 3 animals. For violin plots, circle indicate median, thick vertical lines indicate interquartile ranges and thin vertical lines indicate 1.5X interquartile range.
