Fig. 4: snR107 mediates the Mmi1-Mei2 mutual control. | Nature Communications

Fig. 4: snR107 mediates the Mmi1-Mei2 mutual control.

From: A bifunctional snoRNA with separable activities in guiding rRNA 2’-O-methylation and scaffolding gametogenesis effectors

Fig. 4

a Scheme depicting the different deletion mutants of the mamRNA-U14 locus. b to e Western blots showing the levels of TAP-tagged Mei2 in cells of the indicated genetic backgrounds. An anti-CDC2 antibody was used as loading control. kDa = kilodaltons. f RT-qPCR analyses of mei4 + , ssm4 + , mcp5+ meiotic mRNA and meiRNA levels in cells of the indicated genetic backgrounds (mean ± SD; n = 4 biological replicates; normalized to act1+ and relative to wt). Student’s t-test (two-tailed) was used to calculate p-values (relative to mot2∆). Individual data points are represented by red circles. NS = not significant. Source data are provided as a Source Data file.

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