Fig. 3: Responses of NEMOer variants or G-CEPIA1er to electric field stimulation in rat hippocampal neurons. | Nature Communications

Fig. 3: Responses of NEMOer variants or G-CEPIA1er to electric field stimulation in rat hippocampal neurons.

From: Highly dynamic and sensitive NEMOer calcium indicators for imaging ER calcium signals in excitable cells

Fig. 3

A Typical images showing the fluorescence distribution and responses of NEMOer-c to 25 Hz field stimulation. Pseudocolor cellular images of NEMOer-c before (top left) and after 50 AP (25 Hz) stimulation (bottom left) (scale bar, 10 μm). Enlarged view of the dendrite region (top right), with a white dashed line indicating the position of line scan. Kymograph showing NEMOer-c responses to 25 Hz stimulation within a dendritic region (bottom right) along the indicated line. Scale bar, 2 μm. B Representative tracing of NEMOer-c responses to 25 Hz stimulation, right, statistics (n = 12 cells). Error bar denotes SEM. C Response of NEMOer variants or G-CEPIA1er within dendrites induced by electric stimulation with varies frequencies, with mean response curves shown on the left and statistics shown on the rightmost. Error bars denote SEM. (For stimulation at varied frequencies, G-CEPIA1er, n = 11,11,12,14 cells; NEMOer-f, n = 15, 14, 12, 13 cells; NEMOer-s, n = 11, 13, 12, 13 cells; NEMOer-m, n = 14, 17, 16, 13 cells; NEMOer-c, n = 13, 14, 12, 12 cells). D Typical images showing the individual spine fluorescence and responses of NEMOer-c to 25 Hz field stimulation. Scale bar, 2 μm. Right, statistics. Error bars denote SEM. (G-CEPIA1er, n = 36 spines; NEMOer-c, n = 68 spines).

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