Fig. 5: In vivo NEMOer-f responses to spontaneous SR Ca2+ releasing events in skeletal muscles of zebrafish. | Nature Communications

Fig. 5: In vivo NEMOer-f responses to spontaneous SR Ca2+ releasing events in skeletal muscles of zebrafish.

From: Highly dynamic and sensitive NEMOer calcium indicators for imaging ER calcium signals in excitable cells

Fig. 5

A A schematic depiction of the NEMOer-f in vivo imaging workflow in zebrafish. Created in BioRender. Wang, Z. (2025) https://BioRender.com/k83b811. B A typical sparsely labeled skeletal muscle cell, expressing NEMOer-f. Scale bar, 10 μm. Typical figure captured from 12 cells and 4 zebrafish larvae, showing consistent reproducibility across experiments. C Image montage of time-lapse imaging at 1 Hz. Black circle indicates the ROI for extracting time series in (D). Pink asterisks indicate single Ca2+ events. Scale bar, 10 μm. D Representative time series showing spontaneous SR Ca2+ release indicated by NEMOer-f and G-CEPIA1er signal. Pink and orange asterisks indicate single NEMOer-f and G-CEPIA1er Ca2+ events. E Cumulative distribution of amplitude of NEMOer-f responses (for NEMOer-f, n = 22 events from 12 cells and 4 zebrafish larvae; for G-CEPIA1er, n = 14 events from 9 cells and 4 zebrafish larvae, ****P = 1.97E−7, Welch’s t-test, two-sided).

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