Fig. 3: Impaired LPS-activation dynamics in p30-expressing cells.

a Expression (z-score) of early and late activated genes defined by upregulation in p42 HPC-7 cells after 2 h (early) or 12 h (late) of LPS. Right: significantly enriched GO terms in early and late activated genes (Fisher’s exact test). b Expression (mean z-score) of the early activated genes (top) and late activated genes (bottom) at the different LPS stimulation timepoints (0, 2 and 12 h) in p42 HPC-7 and p30 HPC-7. c LPS-induction (log2 fold change of LPS-treated cells versus untreated cells) of p42-upregulated genes at 2 h and 12 h of LPS stimulation in p42 and p30 HPC-7 cells. d Volcano plot of differential gene expression (p30 vs p42 at 2 h of LPS), showing only genes induced at 2 h in p42 HPC-7 cells. In red, upregulated genes and in blue, downregulated genes, p value < 0.05, log2FC <|0 | . e Expression (mean z-score) of the early activated genes (left) and late activated genes (right) at the different LPS stimulation timepoints (0, 2 and 8 h) in Cebpa^Fl/Fl (WT) and Cebpa^Fl/p30; R26-CreER (p30) macrophages. f LPS-induction (log2 fold change of LPS-treated cells versus untreated cells) of WT-upregulated genes at 2 h and 8 h of LPS stimulation in Cebpa^Fl/Fl (WT) and Cebpa^Fl/p30; R26-CreER (p30) macrophages. g Volcano plot of differential gene expression (p30 vs WT at 2 h of LPS), showing only genes induced at 2 h in WT macrophages. In red, upregulated genes and in blue, downregulated genes, p value < 0.05, log2FC <|0 | . n = 3 biological replicates. c, f Boxplots: center lines represent the median, lower and upper hinges correspond to the first and third quartiles (25th and 75th percentiles). Upper whisker extends from the hinge to the largest value no further than 1.5 * IQR from the hinge (IQR, inter-quartile range). Lower whisker extends from the hinge to the smallest value at most 1.5 * IQR of the hinge. d, g Statistical test used: Wald test, using Benjamini-Hochberg test for adjustment for multiple comparisons.