Fig. 5: Metabolic pathway mutations drive evolutionary rescue and mutualism breakdown.
From: Mutualism breakdown underpins evolutionary rescue in an obligate cross-feeding bacterial consortium
a Growth rates and (b) yields of the mutualism, the prototrophic ancestor, and strains evolved in salinity or PNP. For this experiment, we included one population of the prototrophic strain (gray), six evolutionary replicates derived from salinity stress and five from PNP stress, and the ancestral M and I strains that constituted the mutualism. The data are presented as the mean ± SEM (n = 4). c, d Genes with mutations present in at least two replicate populations or occurring at a frequency > 0.3, represented by different colors (red for salinity and purple for PNP). Color shades indicate the frequency of each mutation, with darker shades indicating higher mutation frequencies. Specific mutations are detailed in Supplementary Tables 2 and 3. Source data are provided as a Source Data file.
