Fig. 2: Enrichment analysis of mouse longevity-associated sites.

a Pathway enrichment analysis of K-to-R replacement sites (hypergeometric test and Benjamini–Hochberg p-value correction by Metascape), and b their corresponding MCODE clusters identified from PPI networks. c, d Similar analysis for K-to-Q replacements. e CC analysis of K-to-R (green) and K-to-Q (blue) replacements. pLOGO of 7 amino acids flanking the mouse total acetylome (f), the longevity-associated acetylation sites replaced to R (g), or Q (h) sites in the mammalian ortholog set. K-to-R = lysine to arginine replacement, K-to-Q = lysine to glutamine replacement. For (f–h), significantly enriched AA are seen between the red lines.