Fig. 5: t-SNE visualization of vector embeddings per cortical layer reveal axes of variation in neuronal morphologies.
From: An unsupervised map of excitatory neuron dendritic morphology in the mouse visual cortex
A t-SNE embeddings per layer colored by percentiles of various morphometric descriptors with example neuronal morphologies along the axis of variation displayed above the embedding. B R2 scores of the six morphometric descriptors (see Fig. 4) per layer showing the strength as predictors of the 32d embeddings. C Spearman’s rank correlation coefficient between morphometric descriptors per layer. Layer 2/3 (blue) Continuum of dendritic morphologies with thinner and less tufted neurons in increasing distance to the pia. Layer 4 (turquoise) Continuation of L2/3 trends with shorter apical dendrites and more atufted cells. Many cells avoid reaching dendrites into L5 (basal bias). Layer 5 (green) Clustering of thick-tufted ET and NP cells. Upper L5 cells resemble L4 cells that avoid reaching into L5, indicating too strict laminar borders. Layer 6 (orange) Continuum with a large morphological diversity, e.g. in cell heights, and existence of horizontal and inverted pyramidal neurons. Source data are provided as a Source Data file.
