Fig. 6: AAV9-mediated CNS transduction induces pro-inflammatory signalling in vivo.

A Study design. AAV9 vector or vehicle control were stereotactically injected bilaterally (B/L) into striatum of adult C57BL/6 J male mice. Striatum was collected 28 days post-injection for snRNAseq processing. Scheme partially created with BioRender. Dzhashiashvili, Y. (2025) https://BioRender.com/e92m679. B UMAP plot of mouse striatum single-nucleus data generated with Seurat. Cells from vehicle and AAV9-treated mice are clustered and plotted in two dimensions using the UMAP dimensionality reduction technique and annotated by cell type. C Donut plot showing the frequency of each cell type per treatment group. D Quantification of the percent of cells positive for GFP mRNA within each cell type. E Dot plots visualizing the enriched up-regulated GSEA terms in different cell types against the Hallmark gene set (Molecular Signatures Database). GSEA was performed on logFC pre-ranked gene lists obtained from gene expression levels of samples transduced with AAV9 compared to vehicle within each cell type. Enriched pathways are ranked according to their normalized enrichment scores. Significance testing was performed with fgsea package in Bioconductor, comparing gene set enrichment scores to a null distribution of random permutations of the gene rankings. P-values were adjusted using the Benjamini-Hochberg correction to control FDR.