Fig. 5: Pan-membrane-protein labeled T cell lymphoma splenocytes travel to the spleen of healthy recipient mice and exhibit material transfer with healthy splenocytes in vivo.

a Schematic illustration of the in vivo material transfer experiment between spontaneously developed T cell lymphomas derived from a SNF5^fl/fl, CD4-Cre^+/- mouse on a C57/BL6 background and bulk splenocytes from a wildtype C57/BL6 mouse. b Injected sulfo-Cy3 (sCy3, green) and sulfo-Cy5 (sCy3, magenta) labeled cells were detected in the recipient splenocytes 24 h post-injection. c Injected sulfo-Cy3 (sCy3, green) and sulfo-Cy5 (sCy5, magenta) labeled cells were also detected in the recipient splenocytes 48 h post-injection. d Membrane protein transfers from labeled-injected cells into recipient splenocytes. Four representative cells (i-iv) showing sulfo-Cy3 (sCy3, green) and sulfo-Cy5 (sCy5, magenta) puncta containing recipient splenocytes lacking membrane labeling. e Immunostaining for CD8α shows membrane transfer from the injected cells to the recipient splenocytes lacking membrane staining of CD8α-FITC. Overlay of fluorescent channels with brightfield (top left), merged fluorescent channels (top right), and individual channels (bottom). Sulfo-Cy5 (sCy5), sulfo-Cy3 (sCy3), and CD8α-FITC immunostaining is shown in magenta, green and cyan, respectively. Representative images from two technical replicates (b–e) from one independent experiment are shown. Scale bars: 10 μm (b, c, left), 2 μm (b, c: right, and d, e).