Fig. 1: Hepatocyte-specific AKAP1 deficiency exacerbates FFD-induced MASH in mice.

AKAP1-Flox and AKAP1-HKO male mice were fed on NCD or FFD for 16 weeks. a Sequential changes in body weight. b Blood glucose levels. c Serum TG and TC levels. d Tissue weight. e Representative images of liver dissected from mice in the indicated groups. Scale bar, 1 cm. f Representative H&E-staining images (left) and NAFLD activity score (NAS) of each group (right). Scale bar, 50 µm. g Representative Oil Red O staining images (left) and quantification of the Oil Red O positive area (right). Scale bar, 50 µm. h Representative Masson’s Trichrome staining images (left) and fibrosis score (right). Upper scale bar, 200 µm; lower scale bar, 50 µm. i Representative immunofluorescence staining images of F4/80 (left) and quantification of the F4/80 positive cells (right). Scale bar, 50 µm. j, k Liver TG and TC levels. l, m Serum ALT and AST levels. For (a), (b) and (d), n = 9 AKAP1-Flox mice and n = 8 AKAP1-HKO mice for the NCD group; n = 12 AKAP1-Flox mice and n = 10 AKAP1-HKO mice for the FFD group. For (c) and (f–n), n = 6 mice per group. Data were expressed as mean ± SEM. Two-way repeated-measures ANOVA followed by Bonferroni post hoc analysis for (a); one-way ANOVA with Bonferroni post hoc analysis for (b–d, f–m). NCD, normal control diet, Source data are provided as Source Data file.