Fig. 2: Restoration of hepatic AKAP1 expression rescues the exacerbated MASH in AKAP1-HKO mice. | Nature Communications

Fig. 2: Restoration of hepatic AKAP1 expression rescues the exacerbated MASH in AKAP1-HKO mice.

From: Hepatic AKAP1 deficiency exacerbates diet-induced MASLD by enhancing GPAT1-mediated lysophosphatidic acid synthesis

Fig. 2

a AKAP1-Flox mice and AKAP1-HKO mice (8 weeks of age) were fed on FFD for 4 weeks, followed by intravenous tail injection of AAV8-TBG-AKAP1 to specifically overexpress and restore liver AKAP1 levels, respectively. After an additional 12 weeks of FFD feeding, mice were euthanized and analyzed. b Western blotting analysis of AKAP1 protein expression. β-actin was used as a loading control. c Body weight. d Serum TG and TC levels. e Liver weight. f Representative H&E-staining images (left) and NAFLD activity score (NAS) of each group (right). Scale bar, 50 µm. g Representative Oil Red O staining images (left) and quantification of the Oil Red O positive area (right). Scale bar, 50 µm. h Representative Masson’s Trichrome staining images (left) and fibrosis score (right). Upper scale bar, 200 µm; lower scale bar, 50 µm. i Representative immunohistochemical staining images of F4/80 (left) and quantification of the F4/80 positive area (right). Scale bar, 50 µm. j, k Liver TG and TC levels. l, m Serum ALT and AST levels. For (b), n = 3 mice per group. For (c–m), n = 8 mice per group. Data were expressed as mean ± SEM. One-way ANOVA with Bonferroni post hoc analysis for all data. Source data are provided as Source Data file.

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