Fig. 1: Map-based cloning of WYMV resistance locus Ym1.
From: A wheat CC-NBS-LRR protein Ym1 confers WYMV resistance by recognizing viral coat protein
A Genetic and physical maps of Ym1 region on chromosome 2DL. The region in pink represents the putative alien introgression fragment. Markers in bold are used to screen recombinants (upper) and define the finely mapped region, respectively. B Recombinant types (designated as N, O, and P) and their WYMV responses. S/WYMV+: WYMV susceptible with abundant coat protein (CP) transcripts in leaves; R/WYMV-: WYMV resistant with no-detectable CP transcript in leaves. C WYMV symptoms (upper) and CP transcripts revealed by RT-PCR (lower) of Yining Xiaomai (YNXM), Yangmai 158 (YM158), and three susceptible YNXM mutants (YNXM-Mut1, YNXM-Mut2 and YNXM-Mut3). The wheat Tubulin gene was used as control. D CP transcripts quantification in the leaves of YNXM, YM158, and three YNXM susceptible mutants by qRT-PCR. ND not detectable. The values of qRT-PCR are the mean ± SD (n = 3 biologically independent experiments). E Diagram showing the nucleotide and amino acid Ym1 mutations in the three WYMV susceptible mutants YNXM-Mut1, YNXM-Mut2 and YNXM-Mut3.
