Fig. 2: Over-expression of Ym1 in WYMV susceptible variety Yangmai 158 enhanced its resistance.
From: A wheat CC-NBS-LRR protein Ym1 confers WYMV resistance by recognizing viral coat protein
A, B Wheat Ym1 gene expression (A) and WYMV CP gene transcript detection (B) in roots and leaves of Yining Xiaomai (YNXM, resistant) and Yangmai 158 (YM158, susceptible) grown in WYMV-infected (+WYMV) nursery and WYMV-free field (-WYMV). Each data is presented as means ± SD (n = 3 biologically independent plants). ***P < 0.001, **P < 0.01, *P < 0.05 based on two-tailed t-tests. N/A not applicable; ND not detectable. C, D Identification of Ym1 T0 transgenic plants in YM158 by qRT-PCR (C) and PCR (D). Results represent the means ± SD (n = 3 technical replicates). ND: not detectable. E, F Quantification of CP transcript abundance by qRT-PCR (E) and disease symptoms observation after artificial inoculation of WYMV clone (F) for three Ym1 positive transgenic plants and two negative transgenic plants in YM158. YM158 was used as the control. Results represent the means ± SD (n = 5 technical replicates).
