Fig. 3: Targeted gene editing of Ym1 in Fielder compromised WYMV resistance.

A Diagrams showing names, positions of deletions in gDNA and cDNA, and corresponding amino acid residues of truncated proteins of the four Ym1 CRISPR-Cas9-edited T₁ mutants in Fielder background. Targets for Ym1 gene editing are shown by vertical red lines. The sgRNA and the protospacer-adjacent motif (PAM) are highlighted in blue and red, respectively, in the wild type (WT) sequences, the deleted nucleotides are shown as “-”, and the numbers of deleted base pairs of each line are shown on the right. B WYMV disease symptoms of seedlings (upper) and leaves (lower) of Ym1 gene-edited mutant plants. C Abundance of WYMV CP transcripts in the leaves of Ym1-edited plants grown in the WYMV nursery. The wheat Tubulin gene is used as the internal control. Values are means ± SD (n = 3 biologically independent plants). ND not detectable. D Visualization of Ym1 in transverse root by RNA in situ hybridization using Ym1 fragment at 3’terminal as a probe. The arrowheads point to the endodermis. The images enclosed in the dashed frames (left) are shown in an enlarged view on the right. st stele, co cortex. Scale bars = 20 μm. E Visualization of WYMY CP RNA in transverse root and basal stem sections from RNA in situ hybridization using WYMV CP as the probe. The arrowheads indicate the endodermis. The images enclosed in the dashed frames (left) are shown in an enlarged view on the right. st stele. co cortex. Scale bars = 50 μm.