Fig. 6: Expi293 derived PDGFA and GDF15 copurify with ISM1 through IMAC.
From: Crystal structure of Isthmin-1 and reassessment of its functional role in pre-adipocyte signaling
a Heat map of the secreted proteins identified by mass spectrometry. The two columns are fraction 1 (F1) and fraction 2 (F2) of Expi293 conditioned media as in Fig. 5d, respectively. Peptides of PDGFA and GDF15 in fraction 2 are highlighted. b 1 µg total protein of fractions 1 (F1) and 2 (F2) of the Expi293 cell-cultured media were loaded for WB detection of GDF15. A representative blot of three independent biological repeats is shown. c 10 µg total protein of fractions 1 (F1) and 2 (F2) of the Expi293 cell-cultured media were loaded for WB detection of PDGFA. A representative blot of three independent biological repeats is shown. d Both fraction 2 (F2) and recombinant purified PDGFAA show potent activity on AKT phosphorylation in 3T3-F442A cells. Data are mean ± s.d. of three independent measurements. A representative blot is shown and quantification of three independent biological repeats are presented as mean ± s.d. Source data are provided as a Source Data file.
