Fig. 2: GRA12 is a virulence factor in the highly virulent South American VAND strain in vivo.
From: GRA12 is a common virulence factor across Toxoplasma gondii strains and mouse subspecies
a Immunofluorescence localisation of the C-terminal HA-tagged GRA12 in the VAND ∆GRA12::GRA12-HA strain. Scale bar represents 10 µm. b Verification of GRA12 expression by anti-HA detection in the VAND ∆GRA12::GRA12-HA strain via western blot. c Survival curve of PWD/PhJ mice infected with either VAND, ∆GRA12 or ∆GRA12::GRA12-HA strains, dose in parenthesis. Significance was tested using a two-sided Mantel-Cox test, N = 5 mice per group. d Number of brain cysts recovered from PWD/PhJ mice infected with VAND ∆GRA12 parasites, N = 5. (i) and immunofluorescence detection of a representative brain cyst stained with DBA. Scale bar represents 50 µm (ii). Source data are provided as a Source Data file.
