Fig. 2: Cryo-EM structure of human HSL.
From: Molecular determinants for the association of human hormone-sensitive lipase with lipid droplets
a The color-coded diagram of human HSL. The catalytic domain (CD) of HSL consists of three subdomains: CD1, CD2, and CD3. b The overall structure of HSL. Residues between N198 and R214, between S324 and Q337, between T491 and A638 (the majority of the regulatory domain), and residues before R4 and after P752 were not built in the structure. c The dimer interface between the N-terminal domain of one protomer and the CD2 subdomain of the other protomer. d The dimer interface between the CD1 subdomain from two HSL protomers. e Alignment of the cryo-EM structure of the catalytic domain of HSL with the crystal structure of the carboxylesterase EST2 (PDB: 1QZ3). The root mean square deviation (RMSD) over 1179 backbone atoms is 1.843 Å. EST2 is colored gray, with the 1-hexadecanesulfonyl group that mimics the substrate of EST2 colored yellow. The catalytic triads of HSL and EST2 are shown as sticks.
