Fig. 8: LD-binding and phosphorylation of HSL are independent processes in 3T3-L1 preadipocytes.
From: Molecular determinants for the association of human hormone-sensitive lipase with lipid droplets
a Subcellular localization of human HSL and its variants in 3T3-L1 preadipocytes. The cells were treated with 1 mM IBMX (a PDE inhibitor) and 10 µM forskolin or the same volume of DMSO control. Wild-type HSL, HSL(FL, 3A), and HSL(FL, 5A), each with an EGFP fused to its C-terminus, were transiently expressed in 3T3-L1 preadipocytes. The cells were imaged as described in Fig. 3e. b Quantification of the EGFP signal of human HSL and its variants on lipid droplets in 3T3-L1 preadipocytes (a total of 15 cells were analyzed from three independent measurements). c Upon treatment with forskolin or DMSO, the protein expression and phosphorylation levels of human HSL and its variants in 3T3-L1 preadipocytes were analyzed using Western blot. The data in a and c, are the results of a representative experiment out of three independent experiments. The data in b represent the mean ± SD of three independent measurements. The data in b were analyzed using the unpaired t-test in Prism to calculate the two-tailed P-values. Source data are provided as a Source Data_Fig. 8 file.
