Fig. 2: Hemispheric differences in the maturation of inhibitory synaptic input in the ACx.
From: Asynchronous development of the mouse auditory cortex is driven by hemispheric identity and sex
a Slices were collected from both hemispheres for age-matched and within-animal comparison of mIPSCs. b Sample traces of mIPSCs recorded at four ages starting from prior to ear canal opening (P10) to the end of the tone critical period (P21). c Cumulative histograms of mIPSC interevent interval from the Left and Right ACx at 4 age groups. d Quantification of developmental changes in mean mIPSC frequency within each hemisphere. (Comparison to the youngest group within each hemisphere. Left ACx: <P12 (n = 12 cells) vs. P12–15 (n = 7), p = ns; <P12 vs. P16–20 (n = 8), p = <0.0001; <P12 vs. >P20 (n = 5), p = 0.0236. Right ACx: <P12 (n = 15) vs. P12–15 (n = 7), p = 0.0038; <P12 vs. P16–20 (n = 9), p = 0.0015; <P12 vs. >P20 (p = 7), p = 0.0055, Kruskal–Wallis, post hoc Dunn’s multiple comparisons test). e Comparison of developmental changes in mean mIPSC frequency between the hemispheres (<P12, p = ns, n = 12 and 15 cells, Left and Right ACx, respectively; P12–15, p = 0.031099, n = 7 and 7 cells; P16–20, p = 0.032951, n = 8 and 9; >P20, p = ns, n = 5 and 7, Multiple Mann–Whitney test, Holm–ŠÃdák correction). f Normalized mIPSCs illustrate developmental changes in the decay time constant. g Quantification of mIPSC decay time constant maturation within each hemisphere. (Comparison to the youngest group within each hemisphere. Left ACx: <P12 (n = 14) vs. P12–15 (n = 5), p = ns; <P12 vs. P16–20 (n = 8), p = ns; <P12 vs. >P20 (n = 5), p = 0.0109. Right ACx: <P12 (n = 18) vs. P12–15 (n = 9), p = 0.0076; <P12 vs. P16–20 (p = 9), p = 0.0318; <P12 vs. >P20 (n = 6), p = 0.0277, Welch’s ANOVA test, post hoc Dunnett’s t-test). h Comparison of developmental changes in mIPSC decay time constant between the hemispheres (<P12, p = ns, n = 14 and 18; P12–15, p = 0.04821, n = 5 and 9; P16–20, p = ns, n = 8 and 9; >P20, p = ns, n = 5 and 6 unpaired Welch’s t-test, Holm–ŠÃdák correction). N = 14 mice. Data are presented as mean values ± SEM; the center of the error bars represents the mean. All statistical tests performed were two-sided.
