Fig. 3: Hemispheric differences in the maturation of excitatory synaptic input in the ACx.

a Voltage clamp recordings were performed sequentially from excitatory neurons in L4 of the Left (left traces) and Right (right traces) ACx (order was randomized between animals). Sample traces of mEPSCs recorded at three ages: P15, P19, and P22. b Cumulative histograms of mEPSC interevent interval from the Left and Right ACx at 4 age groups. c Comparison of the ages to half-peak and max mEPSC frequency maturation between the Left (P15.6–19.2) and Right (P13.9–17.1) ACx indicated by green arrows. Solid lines are polynomial fits to the daily averages of the data collected. d Quantification of developmental changes in mean mEPSC frequency within each hemisphere. (Comparison to the youngest group within each hemisphere. Left ACx: <P12 (n = 5 cells) vs. P12–15 (n = 19), p = ns; <P12 vs. P16–20 (n = 15), p = <0.0002; <P12 vs. >P20 (n = 8), p = ns. Right ACx: <P12 (n = 8 cells) vs. P12–15 (n = 16), p = ns; <P12 vs. P16–20 (n = 16), p = 0.0022; <P12 vs. >P20 (p = 8), p = ns, Kruskal–Wallis, post hoc Dunn’s multiple comparisons test). e Comparison of developmental changes in mean mEPSC frequency between the hemispheres (<P12, p = ns, n = 5 and 8 cells, Left and Right ACx, respectively; P12–15, p = ns, n = 19 and 16 cells; P16–20, p = 0.014, n = 15 and 16; >P20, p = ns, n = 8 and 8, Multiple Mann–Whitney test, Holm–Šídák correction). N = 17 mice. Data are presented as mean values ± SEM; the center of the error bars represents the mean. All statistical tests performed were two-sided.