Fig. 4: Experience-dependent map reorganization is confined to the hemisphere that is actively maturing.

a Male mouse pups were exposed to 7 kHz tone pips between P12–15. Between P35–57 bilateral extracellular recordings were performed. Tessellation maps from one animal displaying recording locations, color-coded by the fraction of spike clusters responsive to 7 kHz. b Examples of individual neuron tone responses (circled in tessellation maps) for the Left and Right ACx. Raster plots, peristimulus time histograms (PSTH), and normalized tuning curves are shown for each neuron. c Percentage of putative individual clusters responding to 7 kHz at a given location (Mann–Whitney, p < 0.0003 violin plots), and pie charts showing the fraction of locations with more than 50% of clusters responsive to 7 kHz (Fisher’s exact test p < 0.0001). d Relationship between brain hemisphere and the responsiveness of individual clusters to 7 kHz tones (solid = responsive, empty = not responsive). Fisher’s exact test (two-sided) indicates that the Right ACx had more clusters responsive to tones close to 7 kHz compared to the Left, p = 0.0015. Left ACx n = 136 and Right ACx n = 150 total recording locations, from 10 and 11 mice, respectively. e Anatomical position of all recording locations organized along the anterior-posterior axis of the ACx. f Female mouse pups were exposed to 7 kHz tone pips between P12–15. Between P35–57 bilateral extracellular recordings were performed. g Examples of individual neuron tone responses (circled in tessellation maps) for the Left and Right ACx. Raster plots, peristimulus time histograms, and normalized tuning curves are shown for each neuron. The same analyses described above (c–e) were performed for recordings in female mice. h Percentage of putative individual clusters responding to 7 kHz at a given location (Mann–Whitney, p = 0.0129 violin plots), and pie charts showing the fraction of locations with more than 50% of clusters responsive to 7 kHz (Fisher’s exact test p < 0.0001). i Fisher’s exact test (two-sided) indicates that in females, the Left ACx had more clusters responsive to tones close to 7 kHz compared to the Right, p < 0.0001. Left ACx n = 58 and Right ACx n = 52 total tone-responsive recording locations from 4 mice. j Anatomical positions of all recording locations organized along the anterior-posterior axis of the ACx. Dashed lines through violin plots depict quartiles. Data are presented as mean values ± SEM; the center of the error bars represents the mean.