Fig. 6: The male Left ACx is not responsive to juvenile tone-rearing history.

a Male mouse pups were exposed to 7 kHz tone pips between P16–19 and P18–21. Between P35–57 bilateral extracellular recordings from the Left and Right ACx were performed under anesthesia using 32-channel, dual-shank silicon probes. b–d (P16–19 exposure), Percentage of putative individual clusters responding to 7 kHz at a given location (Mann–Whitney, p = ns violin plots), and pie charts showing the fraction of locations with more than 50% of clusters responsive to 7 kHz (Fisher’s exact test p = ns). c The relationship between the brain hemisphere and the responsiveness of individual clusters to 7 kHz tones is not statistically significant (solid = responsive, empty = not responsive). The Fisher’s exact test (one-sided) indicates that the Left ACx did not have more clusters responsive to tones close to 7 kHz compared to the Right, p = ns. Left ACx n = 106 and Right ACx n = 86 total recording locations, from 7 animals. d Anatomical positions of all recording locations are organized along the anterior-posterior axis of the ACx. e–g Male mouse pups were exposed to 7 kHz tone pips between P18–21. Between P35–57 bilateral extracellular recordings from the Left and Right ACx were performed under anesthesia using 32-channel, dual-shank silicon probes. e Percentage of putative individual clusters responding to 7 kHz at a given location (Mann–Whitney, p = ns violin plots), and pie charts showing the fraction of locations with more than 50% of clusters responsive to 7 kHz (Fisher’s exact test p = ns). f The relationship between the brain hemisphere and the responsiveness of individual clusters to 7 kHz tones is not statistically significant (solid = responsive, empty = not responsive). The Fisher’s exact test (one-sided) indicates that the Left ACx did not have more clusters responsive to tones close to 7 kHz compared to the Right, p = ns. Left ACx n = 32 and Right ACx n = 30 total recording locations, from 2 animals. g Anatomical positions of all recording locations are organized along the anterior-posterior axis of the ACx. Dashed lines through violin plots depict quartiles. Data are presented as mean values ± SEM; the center of the error bars represents the mean.