Fig. 5: Phenotypic and functional profile of CD8+ T cells after H3N2 challenge. | Nature Communications

Fig. 5: Phenotypic and functional profile of CD8+ T cells after H3N2 challenge.

From: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice

Fig. 5

Previously, rAd-immunized or H1N1-infected mice were challenged with H3N2 (10,000 PFU) 56 days after the initial prime, and lymphocytes from lung tissues were isolated 44 days after the challenge (+ H3N2). One set of mice was not reinfected and served as the control group (- H3N2). Additionally, some lymphocytes were restimulated in vitro using MHC-II- or MHC-I-restricted peptides derived from HA and NP, and ICS was used for the functional identification. A, B Phenotypic differentiation between antigen-specific Teff, TEM, and TCM, and different subsets of TRM. The graphs show the total number of HA533-541- and NP147-155-specific Pent+ CD8+ T cells. C Frequencies of cytokine-specific CD8+ T cells investigated in secondary infected mice (+ H3N2) compared to only primed mice (- H3N2) are shown. A–C Each data point represents an individual mouse, and bars represent the mean of the group (n = 6 mice for rAd and H1N1 (-H3N2), 7 mice for H1N1 (+ H3N2) and 8 mice for rAd (+ H3N2). To compare statistical effects between unchallenged (- H3N2) and challenged (+ H3N2) mice of one group, statistical significances were analyzed by two-tailed Mann-Whitney test (*, p < 0.05; **, p < 0.01; ***, p < 0.001).

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