Fig. 4: 2D array self-assemble from TC-RNA tiles that with two double helices arms.

a The design schematic showcases the five TC-RNA tiles alongside their corresponding 2D array. The orange squares label the blunt end. The red circles indicate the locations of crossovers. b AFM images of assembled arrays of five TC-RNA tiles on mica assembly in 1×TAE Mg²⁺ buffer. c AFM images of assembled arrays of five TC-RNA tiles on mica assembly in 1×TAB Mg²⁺ buffer. The order of AFM images matches the order of RNA tiles shown in panel (a). All the AFM images are taken after annealing from 70 °C to 15 °C for 12 h. All AFM images in this figure were reproduced at least three times, with each experiment conducted independently. The scale bar is 200 nm. The inset image size is 100 nm × 100 nm.