Fig. 5: Adoptive transfer of T-bet+ CD8+ T cells confers ICIs susceptibility in immune-ignorant GCs.
From: T-bet+CD8+ T cells govern anti-PD-1 responses in microsatellite-stable gastric cancers
a Schematic graph of constructing immune-ignorant tumor-bearing humanized mice model. Details are described in the methods section (Created in BioRender. Tang, Shiying. (2025): https://biorender.com/m80m221). b Line plot of tumor sizes overtime (T-betlow + IgG4 vs. T-bethigh + IgG4, P = 0.012; T-betlow + anti-PD-1 vs. T-bethigh + anti-PD-1, P = 1.191e-07; T-betlow + IgG4 vs. T-betlow + anti-PD-1, P = 3.099e-08; T-bethigh + IgG4 vs. T-bethigh + anti-PD-1, P = 2.201e-06. All statistical significance was tested by two-tailed two-way ANOVA). Data are presented as mean ± standard error of the mean (s.e.m.). c mIHC of TME of different groups of humanized mice models. From left to right, T-betlow + IgG4, T-betlow + anti-PD-1, T-bethigh + IgG4, and T-bethigh + anti-PD-1. d Box plots of percentages of T-bet+ CD8+ T cells (P = 0.040, two-tailed paired-wise t-test) or PD-1+ CD8+ T cells (P = 0.026, two-tailed paired-wise t-test) in tumor-infiltrating immune cells. Data was generated by flow cytometer analysis. The bounds of the box span from 25% to 75% percentile, the line represents the median, and the whiskers show the 1.5 Interquartile range (IQR) of the data points. e mIHC images from mice treated with T-bethigh CD8+ T cells + anti-PD-1. On the left, composite image of dapi, CD4, CD8, PD-1, PD-L1, and T-bet were shown. On the right, only dapi, PD-L1, and T-bet were shown. T-bet+-PD-L1+ niche can be observed. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns is abbreviation for not significant.
