Fig. 6: The m6A modifications of DVL2 promote cell proliferation, migration, and polarity formation in LECs of HMC. | Nature Communications

Fig. 6: The m6A modifications of DVL2 promote cell proliferation, migration, and polarity formation in LECs of HMC.

From: TGF-β1-induced m6A modifications accelerate onset of nuclear cataract in high myopia by modulating the PCP pathway

Fig. 6

a, b Protein expressions in cells with different treatments. Cell proliferation (c) and migration (d) in cells with different treatments (n = 6, c: siNC vs. siNC+TGF-β1 p < 0.0001, siNC+TGF-β1 vs. siMETTL3+TGF-β1 p < 0.0001, siNC+TGF-β1 vs. siIGF2BP3+TGF-β1 p < 0.0001; d: siNC vs. siNC+TGF-β1 p = 0.0006, siNC+TGF-β1 vs. siMETTL3+TGF-β1 p = 0.0015, siNC+TGF-β1 vs. siIGF2BP3+TGF-β1 p = 0.0073;). Scale bar: 100 μm. e DVL2 gene expression in cells with different treatments (n = 3, PBS vs. FGF-2 p = 0.0215, PBS vs. FGF-2 + IWP-2 p = 0.3595, FGF-2 vs. FGF-2 + IWP-2 p = 0.0045). f DVL2 protein expression in cells with different treatments (n = 3, PBS vs. FGF-2 p = 0.0018, PBS vs. FGF-2 + IWP-2 p = 0.1168, FGF-2 vs. FGF-2 + IWP-2 p = 0.0178). Cell proliferation (g) and migration (h) of cells with different treatments (n = 3; g: PBS vs. FGF-2 p = 0.0089, PBS vs. FGF-2 + IWP-2 p = 0.1451, FGF-2 vs. FGF-2 + IWP-2 p = 0.0002; h: PBS vs. FGF-2 p = 0.0319, PBS vs. FGF-2 + IWP-2 p = 0.3418, FGF-2 vs. FGF-2 + IWP-2 p = 0.0060). i Primary culture of ARC with FGF-2. Scale bar: 200 μm. Black arrows indicate the original capsule rim; yellow arrow shows cell polarity direction. j Immunofluorescent staining in primary cultures of ARC with FGF-2. Scale bar: 20 μm. k, l Protein expression in cells with different treatments. Cell proliferation (m) and migration (n) in cells with different treatments (n = 6, m: siNC vs. siMETTL3 p = 0.0060, siMETTL3 vs. siMETTL3 + DVL2 OE p = 0.0114, siMETTL3 vs. siMETTL3 +FGF−2 p < 0.0001, siMETTL3 vs. siNC+FGF-2 + IWP-2 p = 0.1302; n: siNC vs. siMETTL3 p = 0.0001, siMETTL3 vs. siMETTL3 + DVL2 OE p < 0.0001, siMETTL3 vs. siMETTL3 +FGF-2 p < 0.0001, siMETTL3 vs. siNC+FGF-2 + IWP-2 p = 0.0024). Scale bar: 50 μm.The band density was normalized to loading control as a ratio for statistical analysis. Level of significance was detected using two-sided one-way ANOVA with Tukey’s multiple comparisons test (c–h, m, n). n = biological replicates, ns. = not significant, ****p < 0.0001, ***p < 0.001, **p < 0.01 and *p < 0.05.

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