Fig. 5: Anxa1-Fpr1 axis regulated the mitochondrial fission of neutrophils.

A GSEA was performed in the Resi; Fpr1^cKO-Con and Resi-Fpr1^cKO groups to compute an enrichment score (ES) for gene set overrepresentation in a ranked mRNA-seq list. A positive ES indicates top enrichment, while a negative ES indicates bottom enrichment. B The ridgeline plot displays GSEA core gene fold changes (positive: upregulated; negative: downregulated). P values were calculated using a permutation test, evaluating the significance of pathway enrichment by comparing the observed enrichment score (ES) with a null distribution generated through random permutations of gene sets. P values are one-sided and adjusted for multiple comparisons using the Benjamini-Hochberg method. C GO-BP enrichment of up/downregulated genes in Resi; Fpr1^cKO-Con VS Resi-Fpr1^cKO, annotated with p values (yellow numbers). P values were calculated using the hypergeometric test to evaluate the significance of gene set enrichment. The test was one-sided, and P values were adjusted for multiple comparisons using the Benjamini-Hochberg method. D Representative electron microscopy images of mitochondria from four groups. Scale bar, 400 nm. The experiment was independently repeated three times. Knockdown group selected using shFPR1-2. E Volcano plot of Resi; Fpr1^cKO-Con VS Resi; Fpr1^cKO mRNA-seq data highlights Bnip3(red) among differentially expressed genes (threshold: p < 0.05, |Log2FC| > 1). n = 3 mice in each group. P values were calculated using the Wald test to evaluate the significance. The test was two-sided. P values were adjusted for multiple comparisons using the Benjamini-Hochberg method. F Representative immunofluorescence staining of HL60 cells from four groups. Knockdown group selected using shFPR1-2. Cells were stained with anti-BNIP3 (green) and DAPI (blue) for nuclei. Scale bar = 15 μm. Right: percentages of BNIP3+ cells by area and number. The experiment was independently repeated three times. Data shown as mean ± SD. P values were calculated one-way ANOVA with Tukey’s multiple comparisons test. G BNIP3 protein levels (WB) in four groups, with α-Tubulin as loading control. H WB analysis of Cytochrome c in mitochondria-free cytoplasmic proteins from four groups, with α-Tubulin as loading control. Source data and exact p values are provided as a Source Data file.